Frontiers in Neurophotonics is an opportunity to meet fellow researchers and students from around the world, discuss and discover the latest advances in live cell optical imaging techniques put in perspective by experimental challenges in the field of neuroscience. The school combines tutorials given by experts in optics, biophotonics, and neuroscience with hands-on experiments involving advanced optical approaches to measure, manipulate and follow molecular events in brain cells and to quantify circuit and synaptic activities in several models.